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. 2009 Jan 5;29(6):1421–1431. doi: 10.1128/MCB.01158-08

FIG. 2.

FIG. 2.

Blimp-1 interacts with LSD1. (A and B) FLAG-tagged Blimp-1 expression vector was cotransfected with an HA-tagged LSD1 expression vector or empty vector into 293T cells for 2 days. Immunoblot (IB) analysis was performed with the indicated antibodies on either lysate (Input), anti-HA immunoprecipitates (IP) (A), or anti-FLAG IP (B). (C) Immunoblot (IB) analysis was performed with the indicated antibodies on either H929 nuclear extract (Input) or IP with the LSD1 antibody or an isotype rabbit IgG (rIgG). Protein molecular weight markers (in thousands) are indicated to the left of each blot. (D) Immunofluorescence images of H929 cells stained with antibodies against Blimp-1, LSD1, and HDAC2. Nuclei were counterstained with Hoechst. Scale bar, 10 μm.