FIG. 7.

Context of histone modifications and LSD1 binding in Blimp-1 target loci in H929 cells. (A and B) Chromatin prepared from H929 plasma cells was subjected to ChIP analysis using an antibody specific to LSD1 (A) or to Blimp-1 (B) or an isotype control rabbit IgG (rIgG). Input DNA was used for normalization. (C to G) ChIP analyses using antibodies against H3Ac (C), H3K4me1 (D), H3K4me2 (E), H3K4me3 (F), or H3K9me2 (G) of chromatin prepared from H929 plasma cells at the indicated gene loci. For each gene locus (C to G), QPCR values obtained from immunoprecipitated samples were normalized to input samples and then were further normalized to the GAPDH locus IP/input ratio, which was set as 1. All data are means ± standard errors of means from three independent experiments.