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. 2009 Jan 5;29(6):1421–1431. doi: 10.1128/MCB.01158-08

FIG. 8.

FIG. 8.

Accessible changes in H3Ac and H3K4me2 chromatin modifications in the CIITA locus upon depletion of Blimp-1 or LSD1. (A) Immunoblot analysis showing efficient depletion of Blimp-1 and LSD1 by Blimp-1i or LSD1i, respectively. (B and C) H929 cells, transduced with Ctrli or Blimp-1 shRNA (Blimp1i) for 3 days, were subjected to ChIP analysis using an antibody against H3Ac (B) or H3K4me2 (C). Immunoprecipitated genomic DNA was subjected to QPCR analysis of the histone modification in each indicated gene locus. (D to H) H929 cells were transduced with Ctrli or LSD1i for 2 days and then subjected to ChIP analysis using anti-H3K4me2 (D), anti-H3Ac (E), anti-H3K4me3 (F), anti-H3K4me1 (G), or anti-H3K9me2 (H). For each gene locus (B to H), the relative increase was calculated as described in the legend for Figure 7. (I) Derepression of CIITA mRNA by depletion of LSD1 in H929 cells. H929 cells were transduced with Ctrli or LSD1i for 2 days and then subjected to RT-QPCR analysis to determine the level of the indicated transcripts. All data are means ± standard errors of the means from three independent experiments.