TABLE 1.
Combinations of siRNA and DNA used for transfection of HeLa cells in AP-2 α rescue assaysa
| Row | siRNA | DNA 1 | DNA 2 | DNA 3 |
|---|---|---|---|---|
| 1 | None | pIRES.GFP | pCI | pCMV.CD4 |
| 2 | None | pIRES.GFP | pCI.Nef | pCMV.CD4 |
| 3 | α-Adaptin | pIRES.GFP | pCI | pCMV.CD4 |
| 4 | α-Adaptin | pIRES.GFP | pCI.Nef | pCMV.CD4 |
| 5 | α-Adaptin | paR-V5.IRES.GFP | pCI | pCMV.CD4 |
| 6 | α-Adaptin | paR-V5.IRES.GFP | pCI.Nef | pCMV.CD4 |
| 7 | α-Adaptin | paR-KR297,340EE-V5.IRES.GFP | pCI | pCMV.CD4 |
| 8 | α-Adaptin | paR-KR297,340EE-V5.IRES.GFP | pCI.Nef | pCMV.CD4 |
a
The various combinations of siRNA duplexes and DNA plasmids used to transfect HeLa cells on day 4 of the α-adaptin RNAi and rescue assays are shown. For each condition, 0.4 μg of DNA 1, 0.3 μg of DNA 2, and 0.3 μg of DNA 3 were used. Cells corresponding to rows 3 to 8 were previously treated with siRNA targeting α-adaptin on day 1 of the assay.