FIG. 2.

Core entry and uncoating in the presence of MG132. HeLa cells on glass coverslips were inoculated for 1 h at 4°C with purified VACV (5 PFU per cell) and then incubated for 3 h at 37°C. After fixation and permeabilization, the cells were stained with mouse MAb 7D11, which specifically reacts with the L1 MV membrane protein, and a rabbit polyclonal antibody specific for the A4 MV core protein, followed by Alexa Fluor 594 anti-mouse IgG and Alexa Fluor 488 anti-rabbit IgG. Cell nuclei were stained with diamidino-2-phenylindole dihydrochloride. An average of 275 cells in each group were counted, and the numbers of cores per cell were plotted.