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. 2009 Jan 7;83(6):2406–2416. doi: 10.1128/JVI.01972-08

FIG. 6.

FIG. 6.

E4orf1 restricts the tumor cell-killing potential of E1B-55K mutant virus dl1520 (ONYX-015) in a Tiam1/Rac1-dependent manner. (A) Cell lines derived from human cervical carcinoma (HeLa), large-cell lung carcinoma (H1299), and malignant glioblastoma (U251, U87) were mock infected or infected with the indicated viruses at a multiplicity sufficient to infect all of the cells. The viability of the cells was determined by trypan blue dye exclusion on the indicated day after infection. Values represent the mean of three independent infections with the standard error of the mean. The asterisk identifies values for MAT2-infected cells that are significantly (P < 0.05) less than the viability of dl1520 virus-infected cells. (B) HeLa cells were infected with the wild-type virus (dl309), the E1B-55K mutant virus (dl1520), or the E1B-55K/E4orf1 double-mutant virus (MAT2) at a multiplicity of 20 and left untreated (circles) or were adjusted to 50 mM HA1077 (squares) or 40 μM NSC2366 (triangles) at 4 hpi. The fraction of viable cells was determined daily for 6 days after infection by trypan blue dye exclusion. The plotted values represent the mean of three independent infections with the standard error of the mean indicated by error bars. The lines represent best-fit curves determined by logistic regression with a quasibinomial model.