FIG. 3.

Influenza virus-induced phosphorylation of RSK2. (A) 293 cells were infected with the PB2-627K virus (MOI of 3.0) or remained mock infected. Three or 5 hours later, cell lysates were immunoprecipitated with anti-RSK2 antibody, followed by Western blot analysis with the indicated antibodies to phosphorylated forms of RSK2. (B) Graphical representation of data shown in panel A. The relative ratios of phosphorylated to nonphosphorylated RSK2 are depicted. Influenza virus infection results in phosphorylation of RSK2. White bars, mock-infected cells; black bars, virus-infected cells.