FIG. 5.

Effect of RSK2 knockdown on influenza virus-induced phosphorylation of PKR. (A) shRSK2 cells and control shGFP cells were infected with the PB2-627K virus at an MOI of 0.01 or 1 and incubated at 33°C. At the indicated times postinfection, cell lysates were prepared and subjected to Western blot analysis with antibodies against PKR, phosphorylated PKR, or β-actin, which served as an internal control. hpi, hours postinfection. (B) Graphical representation of data shown in panel A. The relative ratios of phosphorylated to nonphosphorylated PKR are depicted. Influenza virus infection results in increased levels of phosphorylated PKR in control shGFP cells but not in shRSK2 cells.