FIG. 3.

E7 expression does not affect the spindle assembly checkpoint. Mitotic index of RPE1 cells (A), PHKs containing vector or expressing E7 (B and C), and wild-type (WT) and Rb^−/− MEFs (D). Cells under nocodazole treatment (50 ng/ml) were harvested at various time points, fixed, stained with rat anti-P-His H3 IgG2a and FITC-conjugated anti-rat IgG2a, stained with PI, and analyzed by flow cytometry. The percentage of cells positive for P-His H3 with 4C DNA content was plotted as a function of time (A, B, and D). The increase (relative to interphase cells) of cells positive for P-His H3, with DNA content around 4C, was plotted as a function of time (C). Puro, puromycin. Data represent the means of two to three determinants from a representative experiment of three.