FIG. 6.
E7 expression in cells with 4C DNA content in a G1-like state results in replication. (A) Asynchronous cultures of RPE1-vector cells were incubated in nocodazole (Noc)-containing medium for 48 h. Total protein extracts were resolved by SDS-PAGE and blotted with antibodies against cyclin B, cyclin D1, pRb, and tubulin. (B) RPE1-vector (vec) and E7-expressing cells were treated with nocodazole or DMSO for a total of 72 h. At 70 h posttreatment, the cells were incubated for the remaining 2 h with BrdU. BrdU-positive cells with DNA content of >4C are indicated. Data from a representative experiment (of three) are shown.