FIG. 7.

Absence of Rb in MEFs can lead to polyploidy formation. Asynchronous cultures of wild-type and Rb^−/− MEFs were treated with DMSO or nocodazole (50 ng/ml) for 48 h. Cells were collected, fixed, stained with PI, and analyzed by flow cytometry. Data from a representative experiment (of three) are shown. The percentages of cells with more than 4C DNA content are indicated.