Table 3.

Fold-changes and P-values for comparisons of gene expression in the fracture callus of wild-type (+/+) and myostatin-deficient (−/−) mice two-weeks post-osteotomy using either 18s RNA or GAPDH as housekeeping genes for normalization.

Target sequence	Fold-change, P-value (t-test)
	GAPDH	18s	Combo
Col-I	+0.83, P=0.143	+2.13, P=0.064	+1.77, P=0.124
BMP-2	+0.63, P=0.116	+2.03, P=0.043	+1.55, P=0.055
Runx2	−1.55, P=0.020	−0.03, P=0.940	−2.93, P=0.257
Osx	−1.24, P=0.054	+0.30, P=0.545	−2.37, P=0.377
Sox-5	+1.28, P=0.025	+2.64, P=0.029	+2.43, P=0.022
Sox-9	+0.54, P=0.461	+1.93, P=0.044	+1.45, P=0.144