Figure 5.
Reduced accumulation of various lymphocyte subpopulations in BALF from OVA-challenged RORαsg/sg mice compared with WT mice. BALF was collected and analyzed for the total number of lymphocytes as described in Methods. Various lymphocyte subpopulations were examined by flow cytometry using fluorescein isothiocyanate– or phycoerythrin-conjugated CD4, CD3, CD8, and B220 antibodies. (A) Comparison of total number of lymphocytes. A significant difference in the total number of lymphocytes was observed between OVA-challenged WT and RORαsg/sg mice (*p < 0.0001; n = 25–28 in each group). (B) Comparison of different lymphocyte subpopulations (n = 9–16 in each group). Significant differences were observed in the CD3+ (*p < 0.0001), CD3+CD4+ (∼p < 0.0001), CD3+CD8+ (#p < 0.0001), and B220+ (^p < 0.0001) lymphocytes between OVA-challenged WT mice and OVA-challenged RORαsg/sg mice.