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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Biochemistry. 2008 Nov 25;47(47):12614–12625. doi: 10.1021/bi801475r

FIG. 1. Hsp70 inhibits α-Syn fibril assembly in vitro.

FIG. 1

Monomeric recombinant α-Syn (72 μM) in assembly buffer was incubated in 384-well plates (20 μL per reaction) in the presence of either purified Hsp70 or BSA (7.2 μM). Parallel samples (10 μL each) were removed at various time points and α–Syn fibrillization are monitored with ThT (A) or K114 (B) fluorescence. Substoichiometric quantities of Hsp70 (1:10 relative to monomeric α-Syn) prevented increase of α–Syn amyloid fibrils as indicated by these two dyes. C, Reactions containing α-Syn (72 μM) combined with various concentrations of Hsp70 or BSA (0.72-7.2 μM) were measured for ThT fluorescence after 96 h incubation. Inhibition of α–Syn fibril assembly was observed in a concentration-dependent manner with Hsp70 but not BSA. Data are expressed as mean ± s.e.m. (n = 3-4 per concentration, 2 independent experiments).