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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Biochemistry. 2008 Nov 25;47(47):12614–12625. doi: 10.1021/bi801475r

FIG. 5. Monitoring Hsp70 inhibition of α-Syn488 assembly by FP.

FIG. 5

Reactions containing labeled full-length (72μM) were monitored by measuring FP in samples at various timepoints after the addition of Hsp70 or BSA (7.2μM). Control reactions containing BSA showed large increases in FP corresponding to α–Syn fibrils. However, addition of Hsp70 at the start of the reaction resulted in only a minor increase in FP at 20-24h which remained constant thereafter, likely reflecting formation of a small quantity of α–Syn:Hsp70 complexes.