TABLE 1.
Bacterial strains and plasmids used in the study
| Strain or plasmid | Characteristics | Reference or source |
|---|---|---|
| Strains | ||
| MG1655 tolA | tolA::Kn | 3 |
| DH300 | MG1655 Δ(argF-lac)U169; rprA142-lacZ | 12a |
| DH301 | DH300 rcsF::Cm | 11a |
| DH311 | DH300 rcsB::Kn | 12a |
| LC100 | DH300 tolA::Kn, constructed as DH300 × P1[MG1655 tolA] | This work |
| LC100B | DH300 ΔtolA, constructed by removing the Kn marker in LC100 by expressing the FLP recombinase from pCP20 | This work |
| LC101 | DH301 tolA::Kn, constructed as DH301 × P1[MG1655 tolA] | This work |
| LC102 | DH311 ΔtolA, constructed as LC100B × P1[DH311] | This work |
| Plasmids | ||
| pAA410 | ivy gene of E. coli under PBAD control, pFPV25 backbone, Apr | 6 |
| pAA530 | mliC gene of E. coli under PBAD control, pFPV25 backbone, Apr | 3 |
| pAA100 | gfp gene under PBAD control, pFPV25 backbone, Apr | 2 |
| pCP20 | FLP+ λ cI857+ λpR Rep(Ts) Apr Cmr | 4 |
a
Strain was kindly donated by Sarah Ades, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA.