FIG. 1.

B. subtilis SrtN compensates for the lack of CobB during growth of a S. enterica cobB strain under low-acetate or -propionate conditions. Growth behavior of B. subtilis strains in 10 mM acetate (A and B) or 30 mM propionate (C and D). In panels B and D, the expression of plasmid-borne cobB⁺ and srtN⁺ was by the inclusion l⁺-arabinose (250 μM) in the medium. Open squares, JE4175 (wild type); open diamonds, JE7724 (cobB pta/vector); filled circles, JE7725 (cobB pta/pCOBBB8 cobB⁺); filled inverted triangles, JE7726 (cobB pta/pSRTN1 srtN⁺). On the graph, pcobB⁺ is pCOBB8, and psrtN⁺ is pSRTN1. The growth experiments were performed at least three times, and each experiment was done in triplicate. The data presented here have error bars showing variability; however, in most cases, they are smaller than the symbol. WT, wild type.