TABLE 1.

Strains and plasmids

Strain or plasmid	Relevant genotype	Source or referencea
S. enterica serovar Typhimurium strains
LT2	Wild type
ER2566	F− λ−fhuA2 [lon] ompT lacZ::T7 gene1 gal sulA11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-Tets)2	New England BioLabs
	R(zgb-210::Tn10) (Tets) endA1 [dcm]
LT2 derivative
TR6583	metE205 ara-9	K. Sanderson via J. Roth
TR6583 derivatives
JE4718	cobB1176::Tn10d(tet+) pta102::MudI1734
JE7724	cobB1176::Tn10d(tet+) pta102::MudI1734/pBAD30
JE7725	cobB1176::Tn10d(tet+) pta102::MudI1734/pCOBB8
JE7726	cobB1176::Tn10d(tet+) pta102::MudI1734/pSRTN1
B. subtilis strain
JE8607	SMY (prototroph)
SMY derivatives
JE8610	acuC1::spc+
JE8611	srtN4::spc+
JE9089	acuC1::spc+srtN5::erm+
JE11111	acuC1::spc+srtN5::erm+/pSRTN3
JE11281	acuC1::spc+srtN5::erm+acuA2::kan+
Plasmids
pBAD30	Expression vector, ParaBADbla+	11
pHP13	E. coli-B. subtilis shuttle vector, cat+	A. L. Sonenshein
pDG646	Antibiotic cassette harboring vector for B. subtilis, replicates in E. coli, bla+ in E. coli, erm+ in B. subtilis	BGSC
pDG783	Antibiotic cassette harboring vector for B. subtilis, replicates in E. coli, bla+ in E. coli, kan+ in B. subtilis	BGSC
pDG1726	Antibiotic cassette harboring vector for B. subtilis, replicates in E. coli, bla+ in E. coli, spc+ in B. subtilis	BGSC
pMUTIN4	Pspac promoter harboring vector for B. subtilis, replicates in E. coli, bla+	BGSC
pTYB12	Purification vector, N-terminal self-cleavable intein tag, bla+	New England BioLabs
pACUA2	B. subtilis acuA+ cloned into pTYB12 bla+
pCOBB8	S. enterica cobB+ cloned into pBAD30
pSRTN1	B. subtilis srtN+ cloned into pBAD30 bla+
pSRTN2	B. subtilis srtN+ cloned into pTYB12 bla+
pSRTN3	B. subtilis srtN+ cloned into pHP13
	Under the control of the Pspac promoter, cat+

^aUnless otherwise stated, strains were constructed during the course of this work. BGSC, Bacillus Genetic Stock Center.