FIG. 3.

The ycfS, yebE, ftnB, yccA, yqjA, psd, spy, ppiA, and cpxRA genes are strongly Cpx activated. The promoters of ycfS (A), yebE (B), ftnB (C), yccA (D), yqjA (E), psd (F), spy (G), ppiA (H), and cpxRA (I) were fused to a luxCDABE reporter cassette and transformed into MC4100 (wild type), TR10 (cpxA24) (gray bars), NLP7 [MC4100(pBR322), the vector control (VC) strain for NlpE overexpression (OXP), and NLP8 [MC4100(pLD404)] the NlpE overexpression strain] (white bars). Reporter gene expression was measured at mid- to late log phase (OD₆₀₀ of 0.6 to 0.8) and reported as CPS, corrected for cell density (OD₆₀₀). Actual CPS/OD₆₀₀ values are indicated above each bar. Each assay was performed in triplicate and repeated at least twice. The data from one experiment are reported as average CPS/OD₆₀₀ values. Error bars account for the standard deviations from the means.