FIG. 7.

The ung, ompC, and ppiD genes are weakly or not Cpx regulated. The promoters of ung (A), ompC (B), and ppiD (C) were fused to a luxCDABE reporter cassette and transformed into MC4100 (wild-type), TR10 (cpxA24) (gray bars), NLP7 [MC4100(pBR322), the vector control (VC) strain for NlpE overexpression], and NLP8 [MC4100(pLD404), NlpE overexpression (OXP) strain] (white bars). Reporter gene expression was measured at mid- to late log phase (OD₆₀₀ of 0.6 to 0.8) and reported as CPS, corrected for cell density (OD₆₀₀). Actual CPS/OD₆₀₀ values are indicated above each bar. Each assay was performed in triplicate and repeated at least twice. The data from one experiment are reported as average CPS/OD₆₀₀ values. Error bars account for the standard deviations from the means.