TABLE 2.
Strains and plasmids utilized in this study
| Strain or plasmid | Description | Reference |
|---|---|---|
| Strains | ||
| 2K1056 | F− l −IN(rrnD-rrnE)1 rph-1 Δ(argF-lac)U169 | 61 |
| BW25113 | F− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) LAM− rph-1 Δ(rhaD-rhaB)568 hsdR514 | 1, 20 |
| MC4100 | F−araD139 Δ(argF-lac)U169 rspL150(Strr) relA1 flbB5301 fruA25 deoC1 ptsF25 rbsR | 11 |
| W3110 | F− IN(rrnD-rrnE)1 | 2 |
| JW0450 | BW25113 ybaJ::kan | 1 |
| JW0953 | BW25113 yccA::kan | 1 |
| JW1527 | BW25113 ydeH::kan | 1 |
| JW1835 | BW25113 yebE::kan | 1 |
| JW3065 | BW25113 yqjA::kan | 1 |
| JW3883 | BW25113 cpxR::kan | 1 |
| JW5820 | BW25113 ycfS::kan | 1 |
| MB7 | MC4100(pND12) | 16 |
| NLP7 | MC4100(pBR322) | This study |
| NLP8 | MC4100(pLD404) | This study |
| NLP498 | W3110 cpxA24 | This study |
| NLP621 | 2K1056 cpxR::kan | This study |
| NLP623 | 2K1056 yebE::kan | This study |
| NLP624 | 2K1056 ycfS::kan | This study |
| NLP625 | 2K1056 yqjA::kan | This study |
| NLP626 | 2K1056 ybaJ::kan | This study |
| NLP627 | 2K1056 yccA::kan | This study |
| NLP628 | 2K1056 ydeH::kan | This study |
| NLP629 | 2K1056 dsbA::kan | This study |
| NLP630 | 2K1056 csgB::kan | This study |
| NLP631 | 2K1056 fimA::kan | This study |
| TR10 | MC4100 cpxA24 | 65 |
| TR11 | MC4100 cpxA102 | This study |
| TR51 | MC4100 cpxR1::spc | 65 |
| TR384 | MG1655 zii::Tn10 | This study |
| TR386 | MG1655 cpxR1::spc | This study |
| TR884 | MC4100 λRS88 [spy-lacZ] baeS1::Tn10-cam | 63 |
| Plasmids | ||
| pBR322 | Cloning vector, Ampr Tetr | 8 |
| pCS26 | Low-copy-number cloning vector with a promoterless luxCDABE operon; Kanr | 7 |
| pLD404 | pBR322 with PtetR-nlpE Ampr; overexpresses nlpE | 74 |
| pND12 | pBR322 with PtetR-rpoE Ampr; overexpresses rpoE | 16 |
| pNLP10 | pCS26 with expanded MCS inserted between XhoI and BamHI sites; Kanr | This study |
| pNLP11 | pNLP10 with PdegP::luxCDABE Kanr | This study |
| pNLP12 | pNLP10 with PrdoA dsbA::luxCDABE Kanr | This study |
| pNLP13 | pNLP10 with PppiA::luxCDABE Kanr | This study |
| pNLP15 | pNLP10 with Pspy::luxCDABE Kanr | This study |
| pNLP16 | pNLP10 with PmotAB cheAW::luxCDABE Kanr | This study |
| pNLP17 | pNLP10 with Ptsr::luxCDABE Kanr | This study |
| pNLP19 | pNLP10 with PrpoE rseABC::luxCDABE, Kanr | This study |
| pNLP20 | pNLP10 with PompC::luxCDABE, Kanr | This study |
| pNLP21 | pNLP10 with PsecA::luxCDABE, Kanr | This study |
| pNLP22 | pNLP10 with Paer::luxCDABE, Kanr | This study |
| pNLP23 | pNLP10 with Pung::luxCDABE, Kanr | This study |
| pNLP24 | pNLP10 with ParoK::luxCDABE, Kanr | This study |
| pNLP25 | pNLP10 with PmviM::luxCDABE, Kanr | This study |
| pNLP26 | pNLP10 with Ppsd::luxCDABE, Kanr | This study |
| pNLP35 | pNLP10 with PppiD::luxCDABE, Kanr | This study |
| pNLP43 | pNLP10 with PompF::luxCDABE, Kanr | This study |
| pNLP49 | pNLP10 with PacrD::luxCDABE, Kanr | This study |
| pNLP50 | pNLP10 with PmdtABCD::luxCDABE, Kanr | This study |
| pNLP54 | pNLP10 with PycfS::luxCDABE, Kanr | This study |
| pNLP55 | pNLP10 with PcsgBAC::luxCDABE, Kanr | This study |
| pNLP56 | pNLP10 with PcsgDEFG::luxCDABE, Kanr | This study |
| pNLP57 | pNLP10 with PftnB::luxCDABE, Kanr | This study |
| pNLP58 | pNLP10 with PyccA::luxCDABE, Kanr | This study |
| pNLP59 | pNLP10 with PydeH::luxCDABE, Kanr | This study |
| pNLP60 | pNLP10 with PyqjA::luxCDABE, Kanr | This study |
| pNLP61 | pNLP10 with PycdN::luxCDABE, Kanr | This study |
| pNLP62 | pNLP10 with ParoG::luxCDABE, Kanr | This study |
| pNLP63 | pNLP10 with PybaJ::luxCDABE, Kanr | This study |
| pNLP64 | pNLP10 with PyebE::luxCDABE, Kanr | This study |
| pJW1 | pNLP10 with PcpxP::luxCDABE, Kanr | This study |
| pJW2 | pNLP10 with PcpxRA::luxCDABE, Kanr | This study |
| pVS175 | fliC::lacZ in pRS551, Ampr | 76 |
| pVS182 | flhD::lacZ in pRS551, Ampr | 76 |
| pVS183 | EHEC fliA::lacZ in pRS551, Ampr | 75 |