TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| S. agalactiae | ||
| COH1 | wt clinical isolate | 17 |
| DS107 | COH1 with cspA(S575A) allele | This work |
| DS108 | COH1 harboring pJB107 | This work |
| TOH121 | COH1 bearing cspA::erm allele | 13 |
| L. lactis | ||
| MG1363 | L. lactis host for pMSP3545 | 11 |
| DSL102 | MG1363 harboring pJB102 (wt CspA) | 23 |
| DSL103 | MG1363 harboring pJB105 (S575A CspA) | This work |
| DSL104 | MG1363 harboring pJB106 (D180A CspA) | This work |
| E. coli | ||
| CopyCutter | Used for maintenance of cspA-bearing plasmids | Epicentre |
| JDB1 | CopyCutter harboring pJB103 | This work |
| JDB2 | CopyCutter harboring pJB104 | This work |
| Plasmids | ||
| pCR-XL-TOPO | Vector for cloning PCR products | Stratagene |
| pJB101 | pCR-XL-TOPO bearing wt cspA allele | 23 |
| pJB103 | pCR-XL-TOPO bearing cspA(S575A) allele | This work |
| pJB104 | pCR-XL-TOPO bearing cspA(D180A) allele | This work |
| pMSP3545 | Vector for nisin-inducible gene expression | 5 |
| pJB102 | pMSP3545 with wt cspA allele | 23 |
| pJB105 | pMSP3545 bearing cspA(S575A) allele | This work |
| pJB106 | pMSP3545 bearing cspA(D180A) allele | This work |
| pHY304 | Temp-sensitive vector used for allelic replacements; derivative of pVE6007 (15); Ermr | Craig Rubens |
| pJB107 | pHY304 with cspA(S575A) allele | This work |