TABLE 1.
Genotypes of the bacterial strains used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| BZ31 zce-726::Tn10 | his ΔtrpE5 mukB106 smbB-rne-131 zce-726::Tn10 (λ) | Kindly provided by M. Dreyfus |
| C-1a | E. coli C, prototrophic | 52 |
| C-5684 | Δrnc38::kan | By P1*SK7622 transduction into C-1a (this work) |
| C-5686 | Δrne-131∼Tn10 | By P1*BZ31 zce-726::Tn10 transduction into C-1a (this work) |
| C-5705 | rpsOt::aph | From C-1a/pKD46 by λ Red-mediated recombination with DNA fragment obtained by PCR of pKD13 with primers FG1701 and FG1702; aph cassette inserted at −60 from pnp-p into rpsO transcription terminator (this work) |
| C-5729 | Δpnp-871::cat | From C-5705/pKD46 by λ Red-mediated recombination with pGEX104 insert (this work) |
| C-5731 | Δpnp-871 | From C-5729/pCP20 by FLP-mediated excision of cat cassette (this work) |
| C-5801 | ΔpnpL1001 Δpnp-871::cat | From C-5705/pKD46 by λ Red-mediated recombination with pGEX1001 insert (this work) |
| C-5802 | ΔpnpL1002 Δpnp-871::cat | From C-5705/pKD46 by λ Red-mediated recombination with pGEX1002 insert (this work) |
| C-5803 | ΔpnpL1003 Δpnp-871::cat | From C-5705/pKD46 by λ Red-mediated recombination with pGEX1003 insert (this work) |
| C-5804 | ΔpnpL1004 Δpnp-871::cat | From C-5705/pKD46 by λ Red-mediated recombination with pGEX1004 insert (this work) |
| C-5805 | ΔpnpL1005 Δpnp-871::cat | From C-5705/pKD46 by λ Red-mediated recombination with pGEX1005 insert (this work) |
| C-5806 | ΔpnpL1006 Δpnp-871::cat | From C-5705/pKD46 by λ Red-mediated recombination with pGEX1006 insert (this work) |
| C-5807 | pnpL1007 Δpnp-871::cat | From C-5705/pKD46 by λ Red-mediated recombination with pGEX1007 insert (this work) |
| C-5808 | pnpL1008 Δpnp-871::cat | From C-5705/pKD46 by λ Red-mediated recombination with pGEX1008 insert (this work) |
| C-5809 | pnpL1009 Δpnp-871::cat | From C-5705/pKD46 by λ Red-mediated recombination with pGEX1009 insert (this work) |
| C-5810 | Δpnp-1010t Δpnp-871::cat | From C-5705/pKD46 by λ Red-mediated recombination with pGEX1010 insert (this work) |
| C-5811 | ΔpnpL1011 Δpnp-871::cat | From C-5705/pKD46 by λ Red-mediated recombination with pGEX1011 insert (this work) |
| C-5812 | ΔpnpL1012 Δpnp-871::cat | From C-5705/pKD46 by λ Red-mediated recombination with pGEX1012 insert (this work) |
| C-5813 | ΔpnpL1013 Δpnp-871::cat | From C-5705/pKD46 by λ Red-mediated recombination with pGEX1013 insert (this work) |
| C-5814 | ΔpnpL1014 Δpnp-871::cat | From C-5705/pKD46 by λ Red-mediated recombination with pGEX1014 insert (this work) |
| C-5815 | ΔpnpL1015 Δpnp-871::cat | From C-5705/pKD46 by λ Red-mediated recombination with pGEX1015 insert (this work) |
| C-5851 | ΔpnpL1001 Δpnp-871 | From C-5801/pCP20 by FLP-mediated excision (this work) |
| C-5852 | ΔpnpL1002 Δpnp-871 | From C-5802/pCP20 by FLP-mediated excision (this work) |
| C-5853 | ΔpnpL1003 Δpnp-871 | From C-5803/pCP20 by FLP-mediated excision (this work) |
| C-5854 | ΔpnpL1004 Δpnp-871 | From C-5804/pCP20 by FLP-mediated excision (this work) |
| C-5855 | ΔpnpL1005 Δpnp-871 | From C-5805/pCP20 by FLP-mediated excision (this work) |
| C-5856 | ΔpnpL1006 Δpnp-871 | From C-5806/pCP20 by FLP-mediated excision (this work) |
| C-5857 | pnpL1007 Δpnp-871 | From C-5807/pCP20 by FLP-mediated excision (this work) |
| C-5858 | pnpL1008 Δpnp-871 | From C-5808/pCP20 by FLP-mediated excision (this work) |
| C-5859 | pnpL1009 Δpnp-871 | From C-5809/pCP20 by FLP-mediated excision (this work) |
| C-5860 | Δpnp-1010t Δpnp-871 | From C-5810/pCP20 by FLP-mediated excision (this work) |
| C-5861 | ΔpnpL1011 Δpnp-871 | From C-5811/pCP20 by FLP-mediated excision (this work) |
| C-5862 | ΔpnpL1012 Δpnp-871 | From C-5812/pCP20 by FLP-mediated excision (this work) |
| C-5863 | ΔpnpL1013 Δpnp-871 | From C-5813/pCP20 by FLP-mediated excision (this work) |
| C-5864 | ΔpnpL1014 Δpnp-871 | From C-5814/pCP20 by FLP-mediated excision (this work) |
| C-5865 | ΔpnpL1015 Δpnp-871 | From C-5815/pCP20 by FLP-mediated excision (this work) |
| C-5868 | rne-3071∼Tn10 | By P1*KG241 transduction into C-1a, Tetr selection, screening for TS; presence of rne-3071 confirmed by sequencing |
| C-5869 | rne+∼Tn10 | By P1*KG241 transduction into C-1a, Tetr selection, screening for TS+; presence of rne+ confirmed by sequencing |
| C-5870 | Δpnp-871 rne-3071∼Tn10 | By P1*KG241 transduction into C-5731, Tetr selection, screening for TS; presence of rne-3071 confirmed by sequencing |
| C-5871 | Δpnp-871 Tn10 | By P1*KG241 transduction into C-5731, Tetr selection, screening for TS+; presence of rne+ confirmed by sequencing |
| DH10B | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG | 32 |
| KG241 | rne-3071∼Tn10 | By P1*BZ31 into N3431, Tetr selection, screening for TS; presence of rne-3071 confirmed by sequencing |
| MRE600 | rna | 60 |
| N-3431 | lacZ43 λ−relA1 spoT1 rne-3071 | 18 |
| N-3433 | lacZ43 λ−relA1 spoT1 thi-1 | 18 |
| SK7622 | thyA715 rph-1 rncΔ38::kan | 1 (kindly provided by S. Kushner) |