TABLE 2.
Characteristics of the plasmids used in this study
| Plasmid | Relevant characteristics | Reference |
|---|---|---|
| pAZ76 | pGM743 derivative; harbors 1-380 pnp operon region under T7 promoter | This work |
| pAZ77 | pGM743 derivative; harbors 76-380 pnp operon region under T7 promoter | This work |
| pAZ101 | pGZ119HE derivative; pnp+ | 47 |
| pAZ133 | pAZ101 derivative; harbors Δpnp-833 allele that encodes KHS1-truncated PNPase | 4 |
| pAZ133L1001 | Harbors ΔpnpL1001 Δpnp-833 double mutation | This work |
| pAZ133L1002 | Harbors ΔpnpL1002 Δpnp-833 double mutation | This work |
| pCP20 | Thermosensitive replication, thermoinducible FLP (FRT-specific) recombinase; Ampr Camr | 9 |
| pGEX-1 | Cloning vector; Ampr | 53 |
| pGEX104 | pGEX-1 derivative; harbors pnpL+ Δpnp-871::cat | This work |
| pGEX1001 | pGEX104 derivative; harbors ΔpnpL1001 Δpnp-871::cat | This work |
| pGEX1002 | pGEX104 derivative; harbors ΔpnpL1002 Δpnp-871::cat | This work |
| pGEX1003 | pGEX104 derivative; harbors ΔpnpL1003 Δpnp-871::cat | This work |
| pGEX1004 | pGEX104 derivative; harbors ΔpnpL1004 Δpnp-871::cat | This work |
| pGEX1005 | pGEX104 derivative; harbors Δpnp-1005 Δpnp-871::cat | This work |
| pGEX1006 | pGEX104 derivative; harbors Δpnp-1006 Δpnp-871::cat | This work |
| pGEX1007 | pGEX104 derivative; harbors pnp-1007 Δpnp-871::cat | This work |
| pGEX1008 | pGEX104 derivative; harbors pnp-1008 Δpnp-871::cat | This work |
| pGEX1009 | pGEX104 derivative; harbors pnp-1009 Δpnp-871::cat | This work |
| pGEX1010 | pGEX104 derivative; harbors Δpnp-1010t Δpnp-871::cat | This work |
| pGEX1011 | pGEX104 derivative; harbors ΔpnpL1011 Δpnp-871::cat | This work |
| pGEX1012 | pGEX104 derivative; harbors ΔpnpL1012 Δpnp-871::cat | This work |
| pGEX1013 | pGEX104 derivative; harbors ΔpnpL1013 Δpnp-871::cat | This work |
| pGEX1014 | pGEX104 derivative; harbors ΔpnpL1014 Δpnp-871::cat | This work |
| pGEX1015 | pGEX104 derivative; harbors ΔpnpL1015 Δpnp-871::cat | This work |
| pGM743 | Promoterless pGZ119EH derivative | 62 |
| pGZ119HE | oriVColD Camr | 28 |
| pKD13 | oriRγ bla; harbors the aph (Camr) cassette flanked by FRT sites | 9 |
| pKD46 | oriR101 repA101(Ts) araC araBp-gam-bet-exo Ampr | 9 |