TABLE 4.
Oligonucleotides used in this study
| Oligonucleotide no. | 5′→3′ sequence | Coordinatesa |
|---|---|---|
| FG1080/38b | CTAATACGACTCACTATAGGGGTCACGGTGTGTTGGCC | 206-190 |
| FG1081/20 | GTCGCGAGGATGCGCAGAAG | 79-98 |
| FG1626/40b | CTAATACGACTCACTATAGGGCATTAGCCGCGCGAACCTC | 37-19 |
| FG1627/21 | GAATGATCTTCCGTTGCAGAG | 1-21 |
| FG1682/41b | CTAATACGACTCACTATAGGGGTAGGGTGGAGCTGCTTCGA | 33-52 |
| FG1683/20 | ATTCCGGGGATCCGTCGACC | 1315-1334 |
| FG1701/59c | CTGCGTCGCTAATTCTTGCGAGTTTCAGAAAAGGGGCCTGGCTTTTGAAGCTCACGCTG | −101-61/ 1267-1249 |
| FG1702/60c | CCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAAGATCCCCTTATTAGAAG | −21-60/98-117 |
| FG1802/28 | CATTTGCCCTTAACCGGGCAGGACGCCT | 2299-2308/ 2329-2346 |
| FG1803/30 | CCTGCCCGGTTAAGGGCAAATGGCAACCTT | 2340-2329/ 2308-2291 |
a
Coordinates are from pnp-P +1 if not otherwise indicated.
b
Boldface letters indicate T7 promoter sequence.
c
Boldface letters and coordinates are from the pKD13 plasmid.