TABLE 5.

pnp mRNA abundance and stability in the presence and absence of PNPase

Mutationa	Coordinate		Relative abundanceb		AIc	Half-life (min)
	Start	End	−PNPase	+PNPase		−PNPase	+PNPase
None (pnpL+)	0	0	1.00	0.06	1.00	4	NDd
Δ1001	38	73	1.42	0.81	0.46	5	8
Δ1002	1	75	0.15	0.11	0.28	ND	ND
Δ1011	4	75	0.24	0.21	0.12	ND	ND
Δ1003	1	108	0.57	0.37	0.37	4	8
Δ1012	4	108	0.54	0.52	0.04	ND	ND
Δ1004	1	127	0.37	0.39	−0.06	ND	ND
Δ1013	114	145	0.24	0.05	0.84	5	ND
Δ1014	125	145	0.20	0.04	0.85	ND	ND
Δ1015	132	145	0.20	0.04	0.86	ND	ND
Δ1005	162	167	0.58	0.20	0.70	ND	ND
Δ1006	178	180	0.68	0.18	0.79	ND	ND
Δ1010	2309	2328	0.90	0.13	0.92	ND	ND
1007	147	148	0.53	0.08	0.92	ND	ND
1008	159	159	0.22	0.07	0.74	2	ND
1009	157	157	1.08	0.17	0.90	7	ND

^aMutations are listed in the same order as in Fig. 2.

^bRatio to pnpL⁺ in the absence of PNPase.

^cNormalized AI.

^dND, not determined.