TABLE 4.
Spore used | SD spore prepn | Germination in | Spore germination (%/2 h)
|
|
---|---|---|---|---|
SD | Initial | |||
B. subtilis wt | 300 μM l-val | 1/4 AGFK | <3 | 40 |
B. subtilis wt | 300 μM l-val | AGFK | 65 | 80 |
B. megaterium | 200 μM d-glucose | 250 μM l-pro | <5 | 40 |
B. megaterium | 200 μM d-glucose | 10 mM l-pro | 80 | 90 |
Superdormant spores of B. subtilis wild-type (PS533) and B. megaterium were prepared with moderate germinant concentrations, initial and superdormant spores were germinated with either a moderate or the normal high germinant concentration targeting a different germinant receptor(s) than that targeted in superdormant spore isolation, and extents of spore germination after 2 h of incubation were determined, as described in Materials and Methods. SD, superdormant; wt, wild type; l-val, l-valine; l-pro, l-proline.