TABLE 2.

Primers used in this study

Primer name	Application(s)	Sequence (5′-3′)a
est/lip-F	Amplification and sequencing of cgl upstream region	GAGTATGATGCTAATGTTCCAGG
cgl-diag-R	Amplification and sequencing of cgl upstream region; diagnostic primer for confirmation of pPNG901 integration into genome	CAAAGTTCCGCATCTGCTTAG
cgl-F	cgl insert primer	AATTCTAGATAACCAACAACGGGTGCTGTTTC
cgl-R	cgl insert primer	CGTGAATTCTTATTGATGAACCCGCATCCGTG
cyuC-F	cyuC insert primer	AAATCTAGATAAACAGTTGCAGCATTAACACTAG
cyuC-R	cyuC insert primer	ACTGAATTCTTAAATCATCTTAAGGCCCTTAGTAC
mlp-F	mlp insert primer	GGATCTAGATAAGCAGATAGCACCACAGCAAATG
mlp-R	mlp insert primer	AACGAATTCTTAACCAGTAACAGAACCATTAACG
cgl-diag-F	Diagnostic primer for confirmation of pPNG901 integration into genome	GCATAAGGCGCAAATTCAAGTAAG
cyuC-diag-F	Diagnostic primer for confirmation of pPNG902 integration into genome	GCTCCAGATAATCCTAATGAAC
cyuC-diag-R	Diagnostic primer for confirmation of pPNG902 integration into genome	CTTAACAGTCCCATCCTGTTG
mlp-diag-F	Diagnostic primer for confirmation of pPNG903 integration into genome	CTGTTGGTGTAGCGTCTGTTTTG
mlp-diag-R	Diagnostic primer for confirmation of pPNG903 integration into genome	CATTGGAAGCTGCAATGTTTG
pORI-Em-start-R	pORI28-specific primer for confirmation of plasmid integration into genome	GTTCTCGAGGAACTGTGCTGATTACb
pORI-Em-end-F	pORI28-specific primer for confirmation of plasmid integration into genome	TTCCTCGAGACATGCAGGAATTGACGb
cgl_br11_DIGf	Amplification of cgl probe for Northern blot analysis	TTCTCGTACTGGTAATCCAACG
cgl_br11_DIGr	Amplification of cgl probe for Northern blot analysis	ATGCTAATTGTTCCGCAATCTC
cyuC_br11_DIGf	Amplification of cyuC probe for Northern blot analysis	ATCTTCGGCAGTAAATTCAGAG
cyuC_br11_DIGr	Amplification of cyuC probe for Northern blot analysis	GTGAACAAAAACCAGCCAAA

^aRestriction sites are bolded, and stop codons are underlined.

^bThe restriction site was introduced for other experiments.