TABLE 5.
Virulence of IgaA-deficient mutants of serovar Typhimurium having distinct activation levels of the RcsCDB system
| Strain mixture | Status of the Rcs systema | Competitive indexb
|
|
|---|---|---|---|
| Liver | Spleen | ||
| MD0835-J1 [igaA2::KIXX Δ(apbE′-rcsC′)] × WT | Inactive | 0.63 ± 0.11 | 0.82 ± 0.12 |
| MD0842-J4 [igaA2::KIXX rcsC67′] × WT | Intermediate | 0.65 ± 0.03 | 0.92 ± 0.11 |
| MD0855-J10 [igaA2::KIXX Δ(ompC′-micF)] × WT | High | 0.06 ± 0.02 | 0.07 ± 0.01 |
a
As indicated by the relative levels of expression of the wcaH21::lacZ transcriptional fusion in the MD0835-J1, MD0842-J4, and MD0855-J10 strains (see reference 42).
b
Competitive index values were calculated as described previously (2) after intraperitoneal challenge of BALB/c mice with a dose of 2 × 105 CFU per strain. Liver and spleen were extracted at 48 h postinfection. Shown are the medians and standard deviations corresponding to results from two independent experiments.