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H-NS binds to PR3. EMSA of purified H-NS incubated with fragment A (as detailed in Fig. 1) together with a negative control consisting of a 200-bp fragment spanning the multiple cloning site (MCS) of pBluescript at either 37°C (A) or 20°C (B). The concentrations of H-NS are indicated above the lanes, and lane M contains molecular weight markers. Free DNA and protein-DNA complexes are indicated on the right.
