TABLE 3.

Effects of deletions 3′ to the transcriptional start site on temperature regulation of PR3

Strain	Mean β-Galactosidase activity (Miller units) ± SEMa
	37°C	20°C
P90C(pRS415)	78 ± 7	74 ± 9
P90C(pRSC)	24,643 ± 157	24,800 ± 164
P90C(pRSA)	1,044 ± 85	99 ± 8
P90C(pRSA-1)	577 ± 29	78 ± 5.5
P90C(pRSA-2)	1,375 ± 90	212 ± 15
P90C(pRSA-3)	2,211 ± 79	113 ± 9
P90C(pRSA-4)	2,907 ± 81	150 ± 10
P90C(pRSA-5)	7,886 ± 87	424 ± 23
P90C(pRSA-6)	11,000 ± 85	587 ± 28
P90C(pRSA-11)	12,954 ± 95	2,800 ± 75
P90C(pRSA-12)	18,000 ± 123	17,056 ± 216
P90C(pRSA-17)	20,000 ± 118	21,000 ± 230

^aβ-Galactosidase activity of P90C bearing either the promoter probe plasmid pRS415 or derivatives was assayed at 37°C and 20°C. Plasmid pRSA, pRSC, and pRSA1-17 are described in Fig. 2 and Table 1. Data are the means of three independent experiments performed in triplicate.