TABLE 1.
M. xanthus strains and plasmids used in this work
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| DK1622 | Wild type | 19 |
| SA1708 | ΔrodK (in-frame deletion from positions 307 to 2728)b | 33 |
| SA2800 | ΔrokA (in-frame deletion from positions 31 to 360)b | This study |
| SA2801 | ΔrokB (in-frame deletion from positions 31 to 360)b | This study |
| SA2803 | ΔrokA (in-frame deletion from positions 31 to 360),b ΔrokB (in-frame deletion from positions 31 to 360)b | This study |
| SA2808 | ΔrodK (in-frame deletion from positions 307 to 2728),b ΔrokA (in-frame deletion from positions 31 to 360)b | This study |
| SA2809 | ΔrokC (in-frame deletion from positions 28 to 627)b | This study |
| SA2813 | rokA with mutation in position 166 (exchange of G to A)b | This study |
| Plasmids | ||
| pBJ114 | Vector for constructing in-frame deletions, Kmr and galK+ | 18 |
| pUHE24-2 | Expression vector, Apr | 26 |
| pGEX-2T | Expression vector, Apr | GE Healthcare |
| pSW01 | 1,100 bp (positions −520 to 910 of rokA [positions 31 to 360 deleted])b in pBJ114 | This study |
| pMA16 | 1,100 bp (positions −520 to 910 of rokB [positions 31 to 360 deleted])b in pBJ114 | This study |
| pMA25 | 1,106 bp (positions −244 to 3607 of rodK [positions 307 to 2728 as well as positions 2969 to 3298 of rokA deleted])b in pBJ114 | This study |
| pMA29 | 1,098 bp (positions −522 to 1177 of rokC [positions 28 to 627 deleted])b in pBJ114 | This study |
| pSW33 | 1,430 bp (positions −520 to 910 of rokA [position 166 exchange of G to A])b in pBJ114 | This study |
| pMA134 | pGEX-2T derivative for the expression of GST-RodK-R1 (aa 606 to 717 of RodK) | This study |
| pMA234 | pGEX-2T derivative for the expression of GST-RodK-R1D657N (aa 606 to 717 of RodK) | This study |
| pMA156 | pGEX-2T derivative for the expression of GST-RodK-R2 (aa 731 to 841 of RodK) | This study |
| pMA256 | pGEX-2T derivative for the expression of GST-RodK-R2D782N (aa 731 to 841 of RodK) | This study |
| pMA178 | pGEX-2T derivative for the expression of GST-RodK-R3 (aa 858 to 972 of RodK) | This study |
| pMA278 | pGEX-2T derivative for the expression of GST-RodK-R3D909N (aa 858 to 972 of RodK) | This study |
| pMA10 | pGEX-2T derivative for the expression of GST-RokA (aa 1 to 136) | This study |
| pMA13 | pGEX-2T derivative for the expression of GST-RokAD56N (aa 1 to 136) | This study |
| pMA112 | pUHE24-2 derivative for the expression of His6-RodKKinase (aa 329 to 603) | This study |
| pMA1-4 | pUHE24-2 derivative for the expression of His6-RodKΔsensor (aa 329 to 981 of RodK) | This study |
| pAAR327 | pUHE24-2 derivative for the expression of His6-RodKHDDD (aa 1 to 981) | 33 |
| pAAR350 | pUHE24-2 derivative for the expression of His6-RodKHNDN (aa 1 to 981) | 35 |
| pAAR351 | pUHE24-2 derivative for the expression of His6-RodKHDNN (aa 1 to 981) | 35 |
| pAAR352 | pUHE24-2 derivative for the expression of His6-RodKHNND (aa 1 to 981) | 35 |
| pAAR353 | pUHE24-2 derivative for the expression of His6-RodKHNNN (aa 1 to 981) | 33 |
a
Kmr, kanamycin resistance; Apr, ampicillin resistance; aa, amino acids.
b
All coordinates are relative to the start codon of the relevant gene.