Skip to main content
. 2009 Jan 5;191(6):1777–1786. doi: 10.1128/JB.01437-08

FIG. 2.

FIG. 2.

Characterization of the CDI autoinhibition system. Reversibility and carbon source requirement. (A) CDI+ E. coli LMG194 Δara714 with pDAL728 (DL5263) were prepared and incubated at 37°C with shaking at 225 rpm as described in Materials and Methods for the CDI autoinhibition assay. Cultures were incubated in LBM (open squares), LBM plus arabinose (closed circles), LBM plus maltose (open circles), or LBM plus maltose plus arabinose (closed squares). Maltose (0.2%) was present at time zero, and arabinose (0.2%) was added at 5 h, where indicated. (B) CDI+ DL5263 (squares) and CDI− control E. coli DL5400 cells (triangles) were prepared as described for panel A for the CDI autoinhibition assay. Maltose and arabinose (0.2% each) were added to the indicated cultures at 5 h (closed squares and triangles; shown by arrow on x axis). (C) Extended time course. CDI+ DL5263 was grown and treated as described for panel A in LBM. After 20 h of incubation, cultures were supplemented with 0.2% arabinose (closed circles) or with 0.2% arabinose and maltose (closed squares). A no-supplement control was included (open squares). (D) Inhibition of CDI by Pap pili. CDI+ E. coli cells expressing Pap21 pili (half-shaded squares) or not expressing Pap21 pili (open squares) were prepared as described for panel A in LBM without supplements.