TABLE 3.
Results of Testing for HSV-2 Antibodies by Focus ELISA, Western Blot, and Inhibition Assays
Specimen | HSV-1 ELISA KIT 1 Result | HSV-2 ELISA GEN 1 KIT Index Value | HSV-2 ELISA GEN 2 KIT Result | HSV-2 ELISA GEN 2 KIT Index Value | HSV-2 WB Result | Focus Diagnostics Index Value | Inhibition Assay |
---|---|---|---|---|---|---|---|
1 | - | 1.19 | + | 1.3O | - | 0.68 | a |
2 | + | 3.46 | + | 2.43 | - | 2.3 | >90% |
3 | + | 2.40 | - | 0.36 | - | ||
4 | - | 6.46 | + | 3.20 | + | ||
5 | - | 9.32 | + | 6.86 | + | ||
6 | + | 1.76 | + | 2.34 | - | 2.34 | >90% |
7 | - | 1.81 | - | 0.61 | - | ||
8 | + | 5.16 | + | 4.79 | + |
a, testing by Focus Technologies resulted in a negative index value (<0.90) and sample was therefore excluded from inhibition assays.
HSV-1, herpes simplex virus type 1; HSV-2, herpes simplex virus type 2; ELISA, Focus Technologies enzyme-linked immunosorbent assay; GEN 1, first generation; Focus ELISA kit GEN 2, second-generation Focus ELISA kit; WB, Western blot.