Fig. 3.
(a) Two-layered secondary follicle size increased when cultured in CI or RGD matrices compared to ALG. (b) Only CI and RGD result in a significant increase compared to ALG. (c–e). A representative two-layered secondary follicle on day 0 of culture (c) and on day 8 after culture in ALG (d) or CI (e). Ooc = oocyte, scale bar = 25 µm. Significant differences between treatments are indicated by different letters, p<0.05. Data represented as average±SEM, from two or more independent experiments for each matrix.