Table 3.

Characteristics of plasmid and complexed DNA release from HA-based hydrogels varying in HA:PEG ratio

Solvent	Composition		Plasmid			DNA complexes
	HA (%)	PEG (%)	Initial burst (%)	Initial rate (%/h)	Cumulative release (%)	Initial burst (%)	Initial rate (%/h)	Cumulative release (%)
PBS	1	2	53±5	1.88	76±5	10±2	0.00	1±2
	4	4	40±5	1.75	62±6	5±1	0.00	6±1
	2	4	33±5	0.97	54±6	5±1	0.00	6±1
	2	6	51±6	1.56	59±4	1.3±0.2	0.01	2.1±0.2
	1	6	34±5	1.09	43±2	0.50±0.02	0.01	2.1±0.1
	2	8	24±3	0.43	47±7	2.2±0.4	0.00	2.4±0.4
	1	8	11±3	0.29	20±3	1.1±0.2	0.00	2.4±0.6
HAase	1	2	59±11	2.87	78±4	6.3±0.4	0.00	8±1
	4	4	63±5	2.62	81±4	12.4±0.4	0.00	14±1
	2	4	53±3	1.38	68±2	4.9±0.4	0.00	10±2
	2	6	71±6	2.16	78±5	1.7±0.3	0.00	2.7±0.4
	1	6	40±3	1.47	47±1	0.81±0.06	0.01	1.6±0.1
	2	8	35±2	0.55	41±1	1.2±0.1	0.00	4.8±0.7
	1	8	21±4	0.66	2±4	1.4±0.1	0.00	2.4±0.3

Studies were performed either in PBS alone, or with HAase in PBS.