Figure 4.

The delay of neurulation is concomitant with the delay of Wnt3a expression in Zic2^kd/kd embryo. Lateral (a–c and g–i), dorsal (d–f), and magnified frontal views (j–l) of the E9.0 (a–f) and E10.5 (g–l) Zic2^+/+ (a, d, g, and j), Zic2^kd/+ (b, e, h, and k), or Zic2^kd/kd (c, f, i, and l) littermate embryos stained with Wnt3a probe by whole-mount in situ hybridization. Lines in a–c indicate the rostrocaudal extension of the Wnt3a expression in the dorsal neural tube. Arrowheads in d–f indicate the expression in the dorsal midbrain. The anterior neuropore of Zic2^kd/kd is not closed (f). The arrowheads in g–i and j–l indicate the lumbosacral expression and telencephalic roof plate expression, respectively. ps; primitive streak region.