Mass spectrometric analysis of the trimethylsilyl ethers of the incubated synthetic 3β-hydroxy-24S-methyl-5α-cholesta-8(14),22-dien-15-one substrate and the CYP27A1-formed products collected from HPLC (cf. Fig. 3).
A: mass spectrum of the trimethylsilyl ether of the incubated substrate (3β-hydroxy-24S-methyl-5α-cholesta-8(14),22-dien-15-one) B: mass spectrum of the trimethylsilyl ether of product 1 C: mass spectrum of the trimethylsilyl ether of product 2.