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. Author manuscript; available in PMC: 2009 Feb 26.
Published in final edited form as: J Immunol. 2007 Dec 15;179(12):8122–8127. doi: 10.4049/jimmunol.179.12.8122

Figure 3.

Figure 3

Cytokine production is influenced by the route of immunization and tissue localization on day 8. Lymphocytes from spleen and ILN were isolated on day 8 of i.p. or i.n. LCMV infected mice and after 5 h ± peptide stimulation, stained with Thy1.1, CD8, IFN-γ, and TNF-α. A, Representative staining gating on Thy1.1+ CD8+ Ag-specific cells incubated with GP33–41 peptide. No staining was seen with no peptide (data not shown). B, Percentage of IFN-γ+ by Ag-specific T cells and C, Percentage of IFN-γ+TNF-α+by Ag-specific cells. Data are from three separate experiments.**, p < 0.01;***, p < 0.001.