Nmr1 and Nmr2 repress Eve expression.
Wild-type stage 15 embryonic nerve cords stained for Eve (A–C, H,I), Nmr2 (D,E,H,I), and Nmr1 (F,G). (A) Wild-type expression pattern of Eve within the ventral region of the CNS. (B) Misexpression of Nmr2 in all post-mitotic neurons (ElavGAL4::UAS-nmr2) significantly reduces Eve expression in all Eve-positive neurons (arrow points to location of ELs). (C) Misexpression of Nmr1 throughout the CNS (ElavGAL4::UAS-nmr1) causes a mild decrease of Eve expression (arrow points to ELs). (D,F) Wild-type expression pattern of Nmr2 (D) and Nmr1 (F). (E,G) Expression of Eve throughout the CNS (ElavGAL4::UAS-eve) results in a significant decrease of Nmr2 (E) and Nmr1 (G). (H,I) Eve (red) exhibits a predominantly mutually exclusive expression pattern with Nmr2 (green) in the ventral (H) and dorsal (I) regions of the nerve cord while a single Eve-positive U/CQ neuron (H) co-expresses Nmr2 (arrows).