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. 2009 Jan 15;23(2):181–194. doi: 10.1101/gad.1735109

Figure 1.

Figure 1.

HPV DNA excision and amplification. (A) Schematic diagrams depicting the parental pNeo-loxP HPV-18 and Cre-excised HPV-18 plasmids and PCR primers (thin and thick arrows) to detect total and excised HPV DNA. (B) Ethidium bromide-stained agarose gel of PCR amplification products after 20 and 25 cycles, revealing total HPV DNA (332 bp), excised HPV DNA (450 bp), or β-globin gene (275 bp) from submerged PHK cultures after transfection with pNeo-loxP HPV-18 plasmid with (lanes 1–6) or without (lanes 7–12) the nls-Cre expression plasmid. (M) DNA length markers. (C) Southern blot hybridization for HPV genomic plasmid in day-12 raft cultures. Total DNA from raft cultures of PHKs transfected with pNeo-loxP HPV-18 in the presence (lanes 1,2) or absence (lanes 3,4) of the nls-Cre expression plasmid. Length and copy number standards for EcoR I-linearized parental plasmid (lane 5) or HPV-18 genomic DNA (lanes 6–8).