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. 2009 Jan 15;23(2):181–194. doi: 10.1101/gad.1735109

Figure 5.

Figure 5.

HPV-18 plasmid DNA amplification as it relates to the cell cycle. (A–C) Day-10 raft cultures of normal PHK raft cultures. (D–F) Day-12 raft cultures initiated from PHKs transfected with HPV-18 DNA. Sections were analyzed for cyclin A (green in A,D) or cyclin B (green, B,E), HPV-DNA FISH (red, D–F), and for BrdU (red in A,B, green in C, or yellow Alexa Fluor 647 in D,E). (F) An HPV-18-containing raft culture was pulsed with BrdU for 6 h on day 10 and then chased for 48 h prior to harvest. (G) Day 14 of PHK raft cultures infected with HPV-18 virus at MOI of 800. The section was probed for viral DNA (red), cyclin B1 (green), and BrdU (gold). (H) Patterns of PCNA (red), HPV-18 DNA (gold), and p130 (green) in day-14 raft cultures of PHKs initiated after HPV-18 DNA transfection (top row) or HPV-18 virus infection at MOI of 800 (bottom row). Cellular DNA was revealed by DAPI (blue) in all panels.