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. 2009 Jan 15;23(2):181–194. doi: 10.1101/gad.1735109

Figure 6.

Figure 6.

Complementation of HPV-18 E6*I genome by a retrovirus delivering HPV-18 URR-E6. Raft cultures were harvested over a time course. (A) IHC to detect L1 (reddish brown) on day-18 raft cultures. (Left panel) HPV-18 E6*I-containing cultures. (Middle panel) HPV-18 E6*I-containing PHKs infected with the empty pLC retrovirus. (Right panel) HPV-18 E6*I-containing PHKs trans-complemented with the pLJ HPV-18 URR-E6 retrovirus. Arrowhead points to the boundary between the upper cornified strata and live epithelium below. (B–E) Double-fluorescence detection of p53 (green) and HPV-18 DNA (red) in raft cultures. (B) Day-10 normal PHKs. (C) Day-10 and day-12 wild-type HPV-18-containing PHKs. (D) Day-12 and day-16 HPV-18 E6*I-containing PHKs. (E) Day-16 HPV-18 E6*I-containing PHKs trans-complemented with pLJ HPV-18 URR-E6. Cellular DNA was stained with DAPI (blue).