Skip to main content
. 2009 Jan 15;23(2):208–222. doi: 10.1101/gad.1750709

Figure 4.

Figure 4.

Oct1 S385 and S335 mutations modulate DNA selectivity. (A) Molecular models based on the crystal structure of the Oct1 POU domain dimer bound to MORE DNA (Remenyi et al. 2001). Phospho-Ser 385 and Lys 296 are modeled (red and blue arrows). Bottom image shows predicted Å distance. Images generated using PYMOL (http://www.pymol.org). (B) EMSA using nuclear extracts prepared from Oct1-deficient immortalized MEFs infected with retroviruses encoding wild-type, S385A, S385D, or S385K Oct1. Arrows indicate monomeric (1) and dimeric (2) occupancy, and free probe (P). (NS) Nonspecific. (C) MORE DNA-Oct1 dimer structure showing Ser 335 (red). Lys 435 is also shown. (D) EMSA using nuclear extracts prepared from Oct1-deficient immortalized MEFs infected with retroviruses containing either wild-type, S335A, S335D, or S335K Oct1 cDNAs and probes tagged with Cy5. Arrows indicate monomeric (1) and dimeric (2) occupancy, and free probe (P). The gel was scanned using a Typhoon Imaging system (Molecular Dynamics). (Below) Oct1 Western blot of the input extracts.