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. 2009 Jan 15;23(2):195–207. doi: 10.1101/gad.505709

Figure 2.

Figure 2.

PTB colocalizes and associates with oskar mRNA. (A,B) Stage 5 egg chambers from oskA87/+ (A) and oskA87/Df(3R)pXT03 (B) females expressing GFP-PTB constructs under the control of the mat-α4-tub promoter, and triple-stained for GFP (green in the overlay), DNA (blue, DAPI), and the oocyte cytoplasm marker Orb (red). Bar, 15 μm. (C–E) Distribution of the GFP-PTB protein-trap fusion in wild-type (C), grk2E12/grk2B6 (D), and stauD3 (E) oocytes. (Left) GFP signal. (Middle) Staufen protein. (Right) Overlay. Bar, 60 μm. (F, left panel) RT–PCR amplification of mRNAs recovered in fractions immunoprecipitated from GFP-PTB protein-trap or w control ovarian extracts using anti-GFP antibodies. (Right panel) Amplifications from unbound fractions are used as controls.