Figure 6.

PTB binds to multiple sites along the entire oskar 3′UTR. (A) Affinity pull-down assays comparing the binding of PTB to the whole oskar 3′UTR and to individual 3′UTR subdomains (see Supplemental Fig. S5). The conditions used were similar to those described in Figure 5A. (B) PTB (green) and actin (red) staining showing the accumulation of PTB in the cytoplasm of stage 6 w oocytes, or oocytes either lacking endogenous oskar mRNA (osk^A87/Df) or expressing full-length oskar 3′UTR (see the Materials and Methods for complete genotypes). Bar, 15 μm.