Figure 1.

Radiation-induced apoptosis in Mre11^ATLD1/ATLD1 and Nbs1^ΔB/ΔB CNS. (A) Wild-type (WT), Mre11^ATLD1/ATLD1, and Nbs1^ΔB/ΔB mice at P5 were treated with 8Gy of γ-irradiation (panels c–h) and collected 6 h post-IR. Nonirradiated sections are shown in panels a and b. Immunostaining was performed against active-caspase 3. (Panels a,c,e,g) Images of the retina are at 400× magnification. (Panels b,d,f,h) Images of the dentate gyrus are at 200× magnification. Retinal photoreceptor layer (PR), inner nuclear layer (INL), and plexiform layer (PL) are indicated. Arrows point to immunopositive cells. The number of activated caspase-3-positive cells in the retina (B) or dentate gyrus (C) were quantified; error bars represent standard deviation.