Figure 2.

TGS is suppressed in the ros1nrpd4-1 mutant plants. (A) Luminescence and kanamycin resistance phenotypes. Wild type, ros1, and ros1nrpd4-1 were grown on MS plates and imaged after cold treatment (4°C, 24 h). Wild type, ros1, and ros1nrpd4-1 were grown on MS plates with kanamycin (50 μg/mL), and the pictures were taken after 1 wk. (B) The transcript levels of endogenous RD29A and NPTII transgene in wild type, ros1, and ros1nrpd4-1. Total RNA was extracted from 2-wk-old seedlings with or without cold treatment (24 h, 4°C). COR15A and 18S rRNA were used as cold treatment control and RNA loading control, respectively. (C) Assay for complementation of the ros1nrpd4-1 mutant. The plant expression vector PMDC164 harboring NRPD4 genomic sequence was transformed into the ros1nrpd4-1 mutant plants. The leaves from wild type, ros1, ros1nrpd4-1, and the six T1 individual transgenic lines were used for luminescence imaging after treatment with 200 mM NaCl for 3 h. After the genome DNA was digested with the methylation-sensitive enzyme HaeIII, the amplification of AtSN1 in the six transgenic lines was restored to the same level as that in wild type and ros1.