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. 2009 Feb 1;23(3):318–330. doi: 10.1101/gad.1765209

Figure 4.

Figure 4.

Effect of nrpd4 mutations on DNA methylation, siRNA accumulation, and RNA transcript levels. (A) The nrpd4 mutations reduce DNA methylation at 5S rDNA repeats. Genomic DNA from the indicated genotypes was digested with HpaII (for CG and CHG methylation), MspI (for CHG methylation), and HaeIII (for CHH methylation), followed by Southern hybridization. (B) Detection of RD29A promoter siRNAs, AtSN1 siRNA, siRNA1003, Cluster4 siRNA, AtGP1 siRNA and AtMU1 siRNA, ta-siRNA255, and microRNA171 in the indicated genotypes. The ethidium bromide-stained gel corresponding to tRNA and 5S rRNA was used as a loading control. The positions of size markers are indicated (24 nt or 21 nt). (C) The nrpd4 mutations increase the expression of AtSN1, AtGP1, and AtMU1. The transcript level of the transposons was detected by RT–PCR. TUB8 was used as a control.