Figure 5.

NRPD4 encodes a protein with sequence similarity to RPB4. (A) Diagram of the NRPD4 gene showing the positions of exons (solid boxes), introns, and sites of T-DNA insertions. (B) Sequence alignment of NRPD4 from Arabidopsis and rice and RPB4 from Arabidopsis, rice, yeast, Drosopila, and human. (C) Phylogenetic relationships among NRPD4 and RPB4 proteins. The NRPD4 proteins are from Arabidopsis, rice, and grape, while the RPB4 proteins are from the three plant species and budding yeast, Drosophila, and human. (D) Assay for complementation of the yeast rpb4Δ mutant. The cultures from each of the indicated strains were diluted and spotted onto YPD plates and incubated for 2 d at 28°C or 37°C.